ExoU toxin is a major virulence factor for certain strains of Pseudomonas aeruginosa. It is a cytotoxic phospholipase associated with strains isolated from keratitis. exoU is an acquired gene and is associated with mobile genetic elements. Studies have reported a positive association between fluoroquinolone resistance and possession of exoU. exoU strains have more mutations in quinolone resistance determining regions (QRDR) than non-exoU strains. However, associations between mutations in other drug resistance determining regions and exoU have not been explored. The aim of this study was to determine the relationship between possession of exoU and mutation in drug resistance genes. Antibiotic susceptibility was determined for 22 strains by MIC and MBC. Whole genome sequencing was performed in MiSeq Illumina® platform. Genome assembly was done in the CLC genomics 9.0 and the assembled genome was annotated by RAST 2.0. Mutations were examined in QRDRs (gyrA/gyeB/parC/parE), genes, the multi-drug efflux pump (mexR/nalC), porin protein (oprD), chromosomal beta-lactamase (ampC) and its transcriptional regulators (ampR/ampD). exoU was detected in 8 strains, 7 (88%) of which had at least one mutation in gyrA and parC. In contrast, out of 14 non-exoU strains, mutations in gyrA and/or gyrB was detected only in four (29%) strains. Similarly, all exoU-strains had a mutation in mexR, while only one non-exoU strain showed a mutation in mexR. Furthermore, exoU strains also had higher mutation rates in ampC and ampR than non-exoU. However, there were no differences in the mutation rates in nalC, oprD or ampD. In conclusion, exoU strains showed more mutations in antibiotics resistance genes not only in QRDRs but also in other genes giving resistance to other antibiotics such as b-lactams. This data was supported by their phenotypic resistance patterns. Understanding the association between pathogenic and resistance traits may help to control infections caused by this bacterium