Integrative vectors form a backbone of molecular biology, especially in species whose transformation systems are under development. In Actinomycetes, site specific integrases from Streptomyces phages, in particular the serine recombinases from the bacteriophages φC31 and φBT1, bring specific phage and bacterial attachment sites (attP and attB, respectively) into contact allowing integration of phage DNA into the bacterial genome. As this reaction is unidirectional, vectors containing phage integrase systems have been used in a number of genetic engineering applications. Plasmids bearing the φBT1 integrase have been used to introduce DNA into Streptomyces and Amycolatopsis strains, however, they have not been widely studied in other actinobacterial genera. Here, we show that vectors based on φBT1 can stably integrate into the chromosomes of a range of Nocardia species, and that this integration occurs despite the lack of canonical attB sites in these genomes. Furthermore, we show that a φBT1 integrase based vector can insert at multiple pseudo-attB sites within a single strain, and we also determine the sequence of a possible pseudo-attB motif. These data suggest that φBT1-based integrative vectors can be used to readily insert DNA into the genomes of a range of Nocardia species, however, the precise site of insertion should always be determined empirically to limit the likelihood of off-target effects.