Zinc is an important metal in biology. Zinc deficiency is associated with increased susceptibility to bacterial infections. Since the innate immune system can utilise both zinc starvation and toxicity strategies to combat infections, therefore, bacterial pathogens need stringent control of zinc homeostasis. Here, we investigated the role of zinc import and export in innate immune defense against Group A Streptococcus (GAS), a Gram-positive bacterial pathogen responsible for a wide spectrum of human diseases. In order to determine the importance of zinc import and export during the infection process, we utilised the zinc import (adcA/AII) and export (czcD) deletion mutants in competition with the wild-type strain in in vitro and in vivo virulence assays. We identified that the stage and site of infection differ in the use of zinc as a control strategy.